As a way of preventing the use of a cheat device like Game Genie, additional code was put in place to detect one. If the system discovers any sort of leftover data in its RAM and VRAM, the game will lock-up and display a gray screen. This was done due to the game being released as part of a contest run by MTV.

In August 2017, the game was removed from the Nintendo Switch eShop after its ESRB rating suddenly changed from an E for Everyone to a T for Teen rating. This was due to a poster found in the game's Giant Home level which pictured a massive demon with naked female characters around his crotch area. An updated version was released on the eShop in September 2017 with the poster replaced with another featuring an abstract swirl.

Only a few weeks after the game went on sale, it was pulled from shelves at Toys R Us due to a complaint over the word "lust" printed on the back of the box, which prompted a revision of the game's packaging.

The likeness used for the patient Paulson, E is of the game's art director, Mike Pappas.

Lakitu's Japanese name, 'Jugemu' (ジュゲム); or 'Jugem' as it was romanized in later games of the series, comes from a rakugo folk tale. In the tale, a couple could not think of a suitable name for their newborn baby boy and so the father went to a temple and asked the chief priest to think of a name. The priest suggested several names, but they coulnd't decide on one, so they decided to mix all of those names into one, the final result being:

Concept art shows that Yuri Sakazaki originally had a trio of friends designed in order to help flesh out her character, which consisted of a part-time model, a student, and a 10 year old girl who views Yuri as a big sister of sorts. None of them appear in the game, with the idea being dropped during development.

The Deadpool video game was announced in 2012 at the San Diego comic con, with a teaser trailer. After the convention, on November 27th, 2012. An article appeared written from Deadpool's perspective confirming the game's development and joking that he took over the development of the game.

During the development of Art of Fighting 2, the early original design for Jack Turner bore a slight resemblence to Ray Jackson from the movie Bloodsport.

Early concept art shows that Ryo Sakazaki's stage "National Park" more closely resembled an actual national park, while the final version resembles a farm instead.

Early concept art shows that Eiji Kisaragi's stage had a Japanese motif, whereas in the final version his stage is located in an airport.

During the development of Art of Fighting 2, the early concept behind Eiji Kisaragi was that of a "Short, Mexican Wrestler type character". Additionally, concept art shows that he originally wore a type of mask used by Samurai warriors to hide both their identities and their emotional state as well as to scare the enemy.

Ryuhaku Todoh was considered early on as a playable character, but he was scrapped due to unknow reasons.

Concept art for Temujin shows early character designs as a Karnov-style character, as well as a cowboy.

Blanka could originally return to the ground right after hitting an opponent with his Rolling Attack, allowing the player to follow it up with other moves in a combo. This was ultimately changed for the final release.

In 2017, Nintendo lost a patent lawsuit with Texas-based tech company iLife Technologies Inc. who claimed that the Wii, Wii Remote, and Wii U infringed on their patents. The suit filed by iLife back in 2013 claimed that Nintendo had infringed on their patents which include devices that "contain systems or methods for body movement detection, body movement evaluation, body movement analysis, receiving body movement signals, analyzing body movement signals, responding to body movement signals, and remotely monitoring body movement signals."
The court decided that Nintendo had breached one of their patents and was ordered to pay iLife $10.1 million, less than what they originally wanted which was $150 million. Nintendo is currently seeking to appeal the decision.

The Shovel Knight amiibo figure, which is compatible with the Wii U and 3DS versions of the game, is the first amiibo to be able to function whilst still in its packaging, unaltered. This is because it doesn't include the blocking strip which can be found in every other amiibo packaging. Yacht Club Games made this intentional after receiving feedback from fans who wanted to keep it as a collector's item.

The figure is also the first amiibo to be produced not by Nintendo's own manufacturers. The game itself was also the first non-Nintendo developed title to receive its own amiibo.

In an effort to allow people to play the game who couldn't afford it, the game's developers decided to upload a free version of Darkwood onto the website The Pirate Bay to torrent. Instead of offering free Steam keys, they hoped that doing this would also stop key reselling sites from selling the game, and also hoped for gamers to eventually purchase the game once it was discounted on Steam.

Code, graphical assets, and strings can be found in the game's files for the character Matt the Radar Technician, Kylo Ren's alter ego from the Saturday Night Live sketch. The character was removed from the game late in its development due to legal reasons.

During the 2013 Skullgirls Evo Breast Cancer Donation Drive, anybody who donated $50 USD could request a personal voice message from the voice actors of the game. One of the requests was for Peacock's voice actress, Sarah Anne Williams, to sing the song "Yakko's World" from the popular 90's cartoon Animaniacs.

In the Japanese version, there is an optional sidequest accessible by linking Lunar Knights with Mega Man Star Force through WiFi and entering a modified version of the Konami Code. This side quest is unavailable in all international versions.