Despite MultiVersus being positioned by the gaming press and certain players disappointed with the latter game's lack of content as being a "rival" for having "killed" Nickelodeon All-Star Brawl, the development teams behind both games are fans of one another's work.

When SpongeBob is on-stage in Nickelodeon All-Star Brawl, he automatically activates a code titled "yummerSummon" - this does absolutely nothing, and serves as a comical reference to an infamous unused graphic in SpongeBob Saves the Day! for dataminers to discover. The Yummer graphic is not known to exist in All-Star Brawl.

When asked about why the development team behind Nickelodeon All-Star Brawl chose to include Powdered Toast Man from "The Ren & Stimpy Show" as a fighter, a character from a 30-year-old series who only appeared in 6 out of 52 episodes, Ludosity CEO Joel Nyström responded: "He’s fun. Videogames are fun. It’s a great match."

Nickelodeon originally wanted the developers of Nickelodeon All-Star Brawl to use the 2012 versions of the Teenage Mutant Ninja Turtles. However, they pushed to use the 1987 versions instead despite that iteration never having aired on Nickelodeon at the time, with their reasoning being "we're old".

Following the success of All-Star Brawl, other Nickelodeon crossover games would begin using the 1987 TMNT, including Nickelodeon Kart Racers 3, of which the previous two games used the 2012 TMNT, and Nickelodeon would begin airing the 1987 TMNT animated series on their channel in promotion for the film Teenage Mutant Ninja Turtles: Mutant Mayhem.

In the reveal trailer for Nickelodeon All-Star Brawl 2, a set of gameplay footage from the first installment is shown. This is notable for two reasons, one being that the footage shows off multiple characters not featured in the second game, and that one scene shows a beta version of the Wild Waterfall stage with a smaller waterfall and different colors for the Thornberrys' ComVee. This would lead to a theory that the Wild Waterfall footage was taken from NASB 2 and accidentally inserted into the NASB 1 section of the trailer, which if true would confirm that Lincoln Loud and Leonardo were returning.

This theory was quite easily debunked by Lincoln and Leonardo using their models from the first game while everyone else got revised models, and a KO explosion effect featured in the snippet being different from one shown later in the trailer. Nigel Thornberry's character showcase would later show off a new Wild Thornberrys stage in place of Wild Waterfall, which was shortly followed by a leak showing Lincoln as an NPC cameo on the Loud Castle stage.

Nickelodeon All-Star Brawl does not feature characters from live-action Nickelodeon series. According to Ludosity CEO Joel Nyström, this was due to the developers being unable to envision movesets for live-action characters (i.e. "I'd assume they don't feature a giant, two-ton hammer suddenly produced from your back pocket") and that many of the series did not air in Sweden. When asked about live-action content in an interview, he stated


Despite this, a developer from Ludosity has claimed that a live-action character was considered in the form of Alex Mack from The Secret World of Alex Mack, portrayed by Larisa Oleynik, but this was shot down by Nickelodeon.

Although not explicitly stated, it could also be assumed that live-action characters were off the table for likeness costs, given that All-Star Brawl was initially unable to afford character voices, and that actors involved in Nickelodeon sitcoms have been known to frequently get into extreme scandals that end their careers.

Nickelodeon All-Star Brawl does, however, feature a stage based on the game show Double Dare, and multiple aesthetic references to the iconic Nickelodeon slime which debuted in You Can't Do That on Television!

Alongside Garfield, Rocksteady from Teenage Mutant Ninja Turtles was originally going to be one of the two post-launch playable fighters in Nickelodeon All-Star Brawl - notably without his partner-in-crime Bebop, much like Michelangelo and Leonardo's inclusion without Raphael and Donatello. He was ultimately replaced by Shredder, and all that remained of his inclusion at launch was a placeholder stock image and the codename "hunter".

Rocksteady would eventually become playable in Nickelodeon All-Star Brawl 2.

Competitive players of Nickelodeon All-Star Brawl nickname Rocko's grounded neutral strong attack, "Scream!", "Joe Biden" after a mondegreen interpreting Rocko's scream of "You're fired!" (a reference to the episode Power Trip, where he gets promoted to the boss of the comic book shop he works at and becomes mad with power) as "Joe Biden!"

NASB community manager Thaddeus Crews responded to a now-deleted tweet about the mondegreen with "canon", and later posted a stock render of Rocko with the caption "Joe Biden".

While most other characters in Nickelodeon All-Star Brawl 2 recieved revamped movesets, Jenny's moveset is nigh identical to her moveset from the first NASB aside from inputs that didn't exist in the NASB 1 moveset template. This is due to moveset and gameplay designer Thaddeus Crews considering Jenny's moveset to be one of his favorite kits from the first game.

One of Ren's voice lines on Nickelodeon All-Star Brawl, used for his and Stimpy's grounded down strong attack where he slams down Stimpy as if he were a hammer, is a reference to the streamer CONEY, who in his post-launch NASB gameplay referred to the move as "the STEEEEEEEEEEEMPY", with Ren's second original series voice actor Billy West replicating the cadence of CONEY's scream.

There exists an unused model in Nickelodeon All-Star Brawl of Patrick's head after being shoved through a trombone, in reference to the SpongeBob episode Band Geeks.

If you pause the game on Jellyfish Fields, Gary's eyes will start to bizarrely drift away from one another. When you unpause, they snap back in place.

When used on the Wild Waterfall stage, the Suck-O-Matic can suck up the log platforms, altering the layout of the stage.

Nickelodeon All-Star Brawl's movesets are modified through mathematical functions. Due to the monotony of assigning these values, certain modifiers' values are set to be pi solely as a joke. Thaddeus Crews, who added the instances of pi, stated in a Reddit post about the use of the number:

During development of Nickelodeon All-Star Brawl, Patrick's gravity accidentally ended up being set to 420 in certain unspecified circumstances. Thaddeus Crews stated that it was kept that way as a joke.

If the player holds the button after performing Hugh Neutron's grounded up light and then presses left or right on the stick, they can skid Hugh very slightly. Hugh moves slightly further with every skid, and, as there is no cap to how far the skids can go, he can become so fast that he teleports from one side of the stage to the other with a single skid. This has no practical use in a battle due to the amount of skids required to begin teleporting, as well as the skid count restarting every time Hugh uses the move, but appears to be a deliberate feature implemented by the developers, as every time Hugh skids, he makes a short squeaking noise.

Lincoln Loud's neutral water gun move "The Splasher" had to be reworked during development; the original version of the move stunned opponents and could be cancelled into most actions in the game. This was a flawed move design however, as players could simply cancel the move into a waveland and then perform it again, making an infinite combo. If this move stayed in, Lincoln would have had to be banned from early competitive play, much as Michelangelo would end up being. In the final game, the water gun pushes opponents and cannot be cancelled.

A Twitter post made on the Nickelodeon All-Star Brawl Twitter upon the release of the Garfield DLC shows that his bib alternate costume was originally supposed to be plain white with stains of lasagna tomato sauce instead of clean with red checkers.

In 2023, a set of extremely early Nickelodeon All-Star Brawl screenshots were shared through Discord by a developer, showing an aesthetic closer to Slap City and early versions of SpongeBob, Oblina, Leonardo, The Dump, Jellyfish Fields, and - most notably - Rooftop Rumble, based on Shredder's lair from the 2012 Teenage Mutant Ninja Turtles show instead of the 80s TMNT (despite the 80s turtles having already been decided on this point). Also among the images was Hardcore Chores, Rocko's DLC stage, suggesting that a Rocko's Modern Life character may have been intended for base game.

In the final update of Nickelodeon All-Star Brawl, Patrick's command grabs gained a glitch that made them unable to penetrate through shields, negating their primary purpose. When asked about fixing Patrick's grabs after the launch of the game's sequel, gameplay and character designer Thaddeus Crews responded "I would if I could".