One of Master Hand's attacks has him firing what appear to be golden versions of the Banzai Bill enemies from Super Mario World. These predate the first appearance of a Gold Bullet Bill in the Mario series 3 years later in Super Mario Sunshine. This would also mark one of the few times that one of Master Hand's attacks references another series outside of the Kirby franchise.

The game's official US website refers to the Bob-omb item as "Bomb Soldier".

There is an unused sound of the announcer saying "draw game", suggesting this may be an early name for Sudden Death, or possibly that sudden death originally didn't exist.

According to series creator Masahiro Sakurai in a 2024 video, ideas for the series' name were first brainstormed by team members at HAL Laboratory, while the "Bros." part of the name was picked out by its president Satoru Iwata during a meeting with him, Sakurai, and Ape Inc. founder Shigesato Itoi. Iwata's reasoning for adding Bros. to the title was that despite the game's roster not all being "brothers", he believed the word added the nuance that they were "friends who were settling a little disagreement" instead of just simply fighting.