Crocomire's tongue is a separate enemy that can be killed, but in normal gameplay it is practically impossible to do so as killing it requires the use of 83 Power Bombs. Normally, the game caps off the number of Power Bombs you can have at a time to 50, with the game's speedrunning community deducing that either some type of glitch must be used to acquire more Power Bombs, or you must rely on the 4% drop rate for items dropped from Crocomire's projectiles in order to get more.

According to a Game Shokunin Vol. 1 interview, with game's enemy programmer Yasuhiko Fujii, before the boss fight against Draygon, the player will encounter a group of Evir enemies that do a little “dance”. Their movements actually spell out the words “Keiko Love” in English. This idea came from a girlfriend he dated, name Keiko. Fuiji was busy with work, and couldn't make time for Keiko, so when everyone in the office was asleep at night, he snuck this Easter egg into the game. There were never little Evir dance written in the game's planning documents, so he remembered his heart racing fast as he coded it. However, the team's staff had no knowledge about it and never discovered Yasuhiko's code in the game.

Originally it was planned that, upon death, Samus would scream as her suit would explode to reveal her naked body. However, when the game was released, Samus was given clothes and her screamed was removed.

During the Ceres Space Colony fight with Ridley, hitting him enough will cause him to fly away. At the same time, he will drop the baby Metroid to the ground, before coming back down to pick it up. He will then fly away as normal and the self - destruct sequence begins as usual.

Entering the room that contains the Golden Torizo in Ridley's Lair while holding down the face buttons on the controller will trigger a glitch. The glitch gives the player all of the upgrades, missiles, super missiles, power bombs and energy tanks, minus the Screw Attack, which is obtained after the boss fight.

There's an alternate way to kill Draygon, the boss of Maridia. Destroying the cannons on the wall will cause electricity to flow out of the walls. If you grab on to the electricity with your Grapple Beam while Draygon is holding you, the electricity will flow through Samus and into Draygon, killing her in a few seconds.

At the time of Super Metroid's release, it was the largest file size on a cartridge ever released for the console at 24-megabits.

At the very end of the game, Spoiler:you can save the creatures who tought you the wall jump (the Etecoons) and the other who taught you the super boost jump (the Dachora). You can save them during the self-destruct sequence after beating Mother Brain.

These creatures are present in Metroid Fusion, which takes place after Super Metroid. This time they save Samus from the explosion of the station.

The Etecoons who teach Samus how to wall jump sing the power-up theme before their demonstration.

Samus Aran's appearance in Super Metroid was based on the actress Kim Basinger.