Performing a time stop with the characters Dio or Jotaro after an opponent has been defeated sometimes causes a glitch where the opponent remains frozen for several seconds into the next round. This virtually guarantees another loss for them, as even a single hit while frozen like this causes a large amount of damage.
Contributed by Zpace Jockey
Attachment
Iggy's second special seems to be a reference to Akuma's famous Shungokusatsu from the Street Fighter franchise, also developed by Capcom. Aside from the similar animation, the game's data contains an unused sprite of the kanji for dog (犬,inu), parodying the kanji for heaven (天,ten) that appears during Akuma's Shungokusatsu.
Contributed by Zpace Jockey
Attachment
NSFW - This trivia is considered "Not Safe for Work" - Click to Reveal
After beating arcade mode, a short cutscene plays of the selected character finishing DIO. In the US version DIO flies offscreen, while in the uncensored version of the game DIO instead splits apart and violently explodes, much like his death in the manga.
Contributed by Zpace Jockey
The N'doul bonus stage has a dead Speedwagon Foundation helicopter pilot in the background. The character was removed from the US version of the game because not long after the start the stage, one of N'Doul's Geb water hands attacks the pilot's beeping wristwatch and cuts his hand off.
Contributed by Zpace Jockey
Attachment
Creator Hirohiko Araki is known for having a strong preference for western music, because of this most characters reference a popular band or song in some way. This meant it was necessary to rename some characters to be acceptable for release outside Japan. Examples of name changes include Devo to D'bo and Vanilla ice to Iced, although some references are still included, such as Iced quoting Ice Ice Baby with "You had a problem.... I just solved it." in JoJo's Bizarre Adventure: Heritage for the Future.
Contributed by Zpace Jockey
N'Doul (or N'Dool in the localized version of the game) was originally planned as a playable character, artwork exist that show how he would have fought, along with an Alessi form. It's possible to hack the game to choose N'Doul as a character, showing that a number of assets still exist in the games files, such as an alternate colour, animated life bar portraits, animated super move and VS screen portraits and a cut-in for the "Here Comes A New Challenger!" screen.
Contributed by Zpace Jockey
Attachment
The games data contains sprites for Joseph using his Hermit Purple as a grappling hook, which was likely intended for a mid-story cutscene, Spoiler:when DIO is chasing Joseph around Cairo, which would closer match the anime and manga.
Contributed by Zpace Jockey
Attachment
In the international version, Chaka's blade is coloured brown while in the Japanese release, it is silver. However, Khan and Black Polnareff's swords remain unchanged in both versions,Spoiler:despite the fact that all the 3 characters are technically using the same sword in the storyline.
Contributed by Zpace Jockey
Sho Hayami, the voice actor for Vanilla Ice in the original game, later reprised his role in the animated adaptation of JoJo's Bizarre Adventure: Stardust Crusaders, over 15 years later.
Contributed by Frank Jaeger
Attachment
The character Midler was only shown from the waist-down in the manga, so ##Capcom## asked Araki Hirohiko to redesign her for the game.
Contributed by ProtoSnake
Attachment
Unused sprites for Tower of Gray/Gray Fly can be accessed via the game's debug menu. Information for assembling the sprite is unfinished, and thus uses Jotaro's sprite assembly method instead. The sprites for Gray Fly are mostly finished, which includes various attacks and death animations.

These sprites would later be used in the PSX port, where Gray Fly is an enemy in Super Story Mode.
Contributed by G-Haven