The 1-Up Mushrooms are replaced with hearts most likely becuase, with the Game Boy's lack of color, it would be almost impossible to distinguish a 1-Up Mushroom from a regular Mushroom. This change is also present in the game's sequel.
According to the game's manual, the Honen enemies found in the Muda Kingdom are actually the skeletons of Torion that have been eaten by Tatanga, the main villain of the game.
The game's bonus game music sounds similar to Clu Clu Land's hurry up theme.
The ladder-climbing track is 7.7 seconds long, but only the first 2.2 seconds of the song plays.
The back of the package and the Nintendo eShop description for the game state that Koopa Troopas appear. However, the turtle-like enemies in the game are actually Bombshell Koopas, which were used instead of normal Koopa Troopas because the developers did not have the time to reproduce the Koopa Shell ricochet effect on the Game Boy hardware.
In the instruction manual, Daisy is referred to as "Daisy Princess" instead of "Princess Daisy." This is because of her Japanese name, Deiji-Hime, places the word for Daisy before the word for Princess.
Super Mario Land was the first Mario game created without Shigeru Miyamoto's involvement.