Despite becoming a Microsoft game following the buyout of Rare, the ZX Spectrum version of Jetpac was left intact in the Wii U Virtual Console version of Donkey Kong 64.

The infamous DK Rap was featured in the 2023 film "The Super Mario Bros. Movie", with the chorus portion used to chant Donkey Kong's name as he enters the Great Ring of Kong to battle Mario. Donkey Kong's voice actor Seth Rogen reacted to the DK Rap prior to the film's release, referring to it as "objectively one of the worst rap songs of all time." The original songwriter Grant Kirkhope expressed excitement upon hearing about the song's inclusion in the film, but was dismayed upon seeing that he was not credited as the song's original composer in the movie's credits.

Donkey Kong 64 features a unnamed bat enemy found within certain stages such as Fungi Forest, Creepy Castle and Crystal Caves. This enemy would reappear in future games such as DK: King of Swing but under a seemingly new name: Flipflap.

It turns out that this particular name was already used for this enemy back in the Japanese localization of Donkey Kong 64. In the bestiary within the official Japanese DK64 guidebook, they are named フリップフラップ (Furippufurappu), which of course translates to Flipflap in English.