Over 30 new levels were created for the Game Boy Advance release of the game, Super Mario Advance 4: Super Mario Bros 3. In order to access these new levels, the player requires an e-Reader for Game Boy Advance and the various e-Reader Level Cards. To transfer the levels, the player would require 2 Game Boy Advance systems, the e-Reader, a link cable, and a copy of the game. Because of this, these levels have mostly fallen into obscurity.

Only 10 Level Cards were released in North America, while the other 20 Level Cards were released in Japan.

When the game was re-released on the Wii U Virtual Console and Nintendo Switch Online, all 38 e-Reader levels were unlocked by default as the e-Reader was only supported for the Game Boy Advance.