Not touching any buttons for around 5 minutes will start a "screensaver" playing, which include some of the game's babies and the game's logo flying across the screen.

The Unfluffables are a parody of Bambi and Thumper from the James Bond movies. Their species is a reference to the Disney movie, Bambi.

In the original Dreamcast release, it was possible to climb the fencing in the Undermill with Juliette. This leads down to a scrapped part of the level, where Juliette's original training level was supposed to take place. The ability to climb the fence was removed and the gap was raised in all other versions.

At one stage during development, bosses would have names for each of their attacks, which would be displayed on-screen. For example, Gwynth's main attack was known as 'Roof Breath'.

The lead designer Jeff Lewis and lead programmer Mark Craig spent over 200 hours converting the Dreamcast version of the game to iOS.

For a long time, Fur Fighters was known as 'Furballs'. The name was changed fairly late in development to avoid similarities with a US cartoon character.

Tweek is listed as being born in Royston Vasey. This fictional town is the setting of British comedy series The League of Gentlemen.

In Dinotopolis there is a box with the Shrimp enemy which is labelled Sea Minkeys. This is a reference to Sea-Monkeys, a brand name for a kind of brine shrimp, sold as pets.

Although the game lists 2000 Gold Tokens, it is actually possible to obtain more by collecting the Tokens in each Hubworld.

In The Bad Place, all standard enemies with costume changes can be found in the level except the Peacock. The Peacock does however have a model for the level found within the game's coding, though it goes unused.

Several posters on various levels in the game feature the name "West Side Bears". This is a reference to "West Side Story".

In the iOS version of the game, Viggo on Glass, the 'World Quack Centre' was renamed to 'Empire Quack Building'. This was likely to avoid any references to the World Trade Center and the 9/11 terrorist attacks.

There are two posters in Lower East Quack - 'Ducks' and 'The Thin Red Lion', referencing the two stage plays 'Cats' and 'The Thin Red Line'.

In Gwynth's family portrait there are 15 baby dragons shown (excluding Tweek). However, in the game, there are only 14 baby dragons that can be rescued.

In Space Station Meer there is an alien that attempts to attack the player before a fox known as "31st Century Fox" stops him, complaining about copyright issues. This is a reference to the Alien franchise which is held by 20th Century Fox.

The secret Fluffmatch level, Land of the Happy Moles, is based on Bizarre's former office, even containing photographs from the office itself.

In New Quack City, several of the store names are based off actual stores. For example:
• QuackDonalds - McDonalds
• W.H. Hoot - W.H.Smith
• Coo-op - Co-op
• Tweet-11 - 7-Eleven

In the Fluffmatch 20,000 Fluffs B.C., there is a box of matches called Viggo's Glory. This is a reference to England's Glory matches.

In Dinotopolis is a book entitled 'Charlie Darwinosaurus - Survival of the Biggest'. This is a reference to Charles Darwin's book, Survival of the Fittest.
Underneath it is another book called 'Humanic Park', a reference to Jurassic Park.

The Gamequack store in World Quack Centre contains a number videogame parodies, including Resident Weevil (Resident Evil), Shenmoo (Shenmue) and Metropolis Sheep Racer (Metropolis Street Racer).

General Viggo's pet human, Fifi, was originally going to speak.

On the 27th page of the manual for the PC and Dreamcast versions, an unreleased sequel only referred to as Fur Fighters II is mentioned. In the PS2 release, the mention is dropped from the manual.