In Chapter 1: New Arrivals, when playing on a completed save, if the player breaks the light air vent on the left side from a different room from the Flight Lounge, a Necromorph will suddenly appear, but will not trigger a cutscene, causing the game to glitch and allowing Isaac to humorously lead the Necromorph around the Flight Lounge as the crew does not notice the intruder.
Contributed by ProtoSnake
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The background images of the title screen and menus are actually real footage of a dead goat. The development team bought a goat from a local butcher and spent the day taking various shots of the carcass and innards to further the unsettling atmosphere of the game.
Contributed by KidDivinegon
Completing the asteroid gunning section in Chapter 4 with over 50 percent of the ship's shield still remaining gets you a trophy/achievement titled: "Don't get cocky, kid."

This is a line spoken by Han Solo in Star Wars Episode IV: A New Hope during a similar scene.
Contributed by Boyobmas
If the main menu screen is left up for long enough, a cinematic of the game's death scenes will play to a very startling rendition of the song "Twinkle Twinkle Little Star."
Contributed by KidDivinegon
On the options screen, some symbols can be seen on the left side of the screen. These symbols are Unitology symbols and translated hint Spoiler: that the "Red Marker is man made."
Contributed by KidDivinegon
To come up with ideas for the Necromorph enemies, developers studied the carnage of car wrecks. Ideas for the scares were conceived by watching various horror movies.
Contributed by KidDivinegon
Dead Space started production as an Xbox game. The game was later re-purposed as a next-gen game ultimately releasing on the Xbox 360 and PS3.
Contributed by KidDivinegon
At the end of the game, it is revealed that Spoiler:Nicole Brennan committed suicide before you even started your adventure. If you look back at the first letter of each chapter in the game, the letters spell "Nicole is Dead".
Chapter 01: New Arrivals
Chapter 02: Intensive Care
Chapter 03: Course Correction
Chapter 04: Obliteration Imminent
Chapter 05: Lethal Devotion
Chapter 06: Environmental Hazard
Chapter 07: Into the Void
Chapter 08: Search and Rescue
Chapter 09: Dead on Arrival
Chapter 10: End of Days
Chapter 11: Alternate Solutions
Chapter 12: Dead Space
Contributed by DidYouKnowGaming
There's an achievement called "Live With the Hot Ones" for setting enemies alight with the flame thrower. This is a direct reference to an obscure line in the EA code of conduct. Every employee at EA is contractually obligated to "Live With the Hot Ones". No one had any idea what it meant, so it became something of an in-joke at EA.
Contributed by DidYouKnowGaming