On the song "Wanted: Dead or Alive," If you do well enough to have the audience sing along with your band, they will mess up during the chorus by singing an extra "wanted," even though that happens only once in the song. This derives from how fans of Bon Jovi would do the same thing at Bon Jovi concerts.

After the South Park episode "Whale Whores" included a segment where the characters played Lady Gaga's "Poker Face" on Rock Band (which was not in the game), Harmonix released the song as DLC, as well as a special cover version of the song performed by Eric Cartman.