In the DS, both Japanese versions of the PlayStation and Saturn release, and some PC versions of the first Resident Evil game, the cutscene after encountering the first zombie includes a scene where Kenneth's head falls to the floor and rollls towards the camera. The head scene was removed from the other versions, even though his head is still detached from his body.

In later games containing the same scene, such as Resident Evil (2002) and Resident Evil: The Umbrella Chronicles, Kenneth's head remains attached to his body.

The DS version's Multicard Play is the first time in the series nearly all canon S.T.A.R.S. members are playable and is also the only way to see Kenneth Sullivan, Forest Speyer, and Richard Aiken without any damage done to them in-game. The only missing S.T.A.R.S. members are Brad Vickers, Edward Dewey, and Joseph Frost, most likely because they never had actual character models in the game.

At the grave beneath the second Yawn battle, there is a new angle of the tombstone that reads 'LISA TREVOR'. This references the mutant girl of the same name who first appeared in the 2002 remake of Resident Evil.