The reload animation for the Handcannon is different before and after it is fully upgraded. Before the weapon is fully upgraded, Leon will reload the Handcannon with three bullets. However, if it is fully upgraded, the reload animation will change to Leon reloading the Handcannon with a speed loader.

In the Meta Quest release, while hiding in a dumpster, Ashley's model will be in a frozen state. However, her hair and clothing will still be affected by the wind.

In the Meta Quest release of the game, in Chapter 4-1 in the fire dragon room, after the player has taken down the last Los Illuminados member on the fire dragon, some of the hitboxes where the chains once were can be aimed at, but are completely invisible.

In the Meta Quest release of the game, in Chapter 2-3 when the player is given the option to take the path with the second El Gigante, if Ashley is grabbed by El Gigante, and the player does enough damage to make it drop Ashley, she will lose her right eye model until the player has left the area.

When playing as either Krauser or HUNK in The Mercenaries game mode, their respective theme music is the Fourth and Eighth Mission Tunes from the game P.N.03, which was also developed by Capcom.

On a bulletin board in the room with security monitors that's shut off when Leon sees Ashley, there's an image of a hamster-like monster. This is actually taken from the cover art of Bloodwych, a 1989 RPG dungeon crawler, originally developed for the Commodore 64 and eventually released on half a dozen 8-bit home computer systems.

In the Japanese release of the game, the sections in Chapter 3-4 where you play as Ashley feature fixed, dynamic camera angles akin to Resident Evil Code: Veronica that are not found in the international releases of the game.

After the helicopter scene with Mike you can see a person hiding behind a pillar in the distance past the ruins and rubble when using the sniper rifle with an extra scope. It is unknown who this person is, though it is likely it is someone from the development team or someone related to them.

Though largely unused in-game, the characters Hunnigan and Mike have complete character models that can only be seen through camera hacks.

Hunnigan appears in-game during her radio conversations with Leon, where only her head and shoulders are visible. However, moving the camera reveals that these conversation are simply full character models of Leon and Hunnigan sitting next to each other behind a radio texture. Additionally, Hunnigan's model contains multiple layers of clothing, including a layer for both her blazer and blouse. Digging through the game's files also reveals a texture for a white blouse with a black bra and skin clearly visible underneath it.

Likewise, Leon's pilot Mike is never seen outside of his helicopter in-game. Moving the camera into the helicopter during his scenes, however, reveals a full character model inside of the cockpit. Oddly, the character model has yellow eyes, a trait usually associated with the game's monsters.

The lurching man is an unused creature designed for Resident Evil 4, which was planned to be able to change forms. Unfortunately it never got past the design phase.

The "Electric Man" was a creature designed for Resident Evil 4, although it didn't make it past the design phase.

The "Living Doll" is an unused enemy from the second prototype, (also known as the "Hallucination" build), it appeared in the E3 2003 demo for Resident Evil 4. Which at the time featured Leon being attacked by hallucinations.

Concept art exists for an unused "Parasite Woman" enemy, in both an "A-Type" and "B-Type". The "A-type" shows her bound in a straitjacket, and one of her arms has grown to gargantuan lengths. It would appear that her design was reused for the J'avo in Resident Evil 6.
The "B-Type" variant has no arms free at all, and her head has transformed into a sharp-toothed monster.
The third piece of concept art is an unbound woman in a tattered dress, armed with a shotgun.
The final design, merges the earlier designs - an unbounded woman with two inhuman arms growing next to normal ones, while a monster's face projects from the side of her head.
The creature's straight-jacket concept shares an uncanny resemblance to the Nosferatu From Resident Evil Code: Veronica X and Lisa Trevor from the Resident Evil remake.

Any instances of decapitation were strictly removed from the Japanese release.

During the segment of the game when playing as Ashley, it is possible to open a door into a Zealot to stun them and then perform Leon's suplex move on them. This glitch is only present in the GameCube version of the game, and was edited out in all other versions.

Nuby Tech, a gaming peripheral company, created a chainsaw-shaped controller inspired by the Chainsaw Man enemy in Resident Evil 4. Separate versions were made for the GameCube and PS2. However, it didn't enhance the playing experience, and was criticized for its poor button layout.

The gun Broken Butterfly has a description which references the film Sudden Impact. In the movie, a character says "Go ahead, make my day" while holding a revolver. The description for the Broken Butterfly reads: "This will make anyone's day."

The title menu for Ada's Separate Ways Campaign was likely inspired by the movie poster for La Femme Nikita.

When the player attempts to look up Ashley's skirt she will exclaim "UGH! You PERVERT!" Or "HEY! What are you looking at!" and cover herself. Humourously, this will trigger if Leon's head ends up directly under her skirt in any situation including if he is knocked down by enemies.

If Ashley is wearing any of her alternate costumes, this animation will not trigger.

During the standoff with the Ganados in the cabin, shooting Luis enough times will trigger a cutscene where he will shoot and kill Leon, resulting in a game over.

Trying to reload the Chicago Typewriter (which has infinite ammo by default) while wearing the Leon's Mobster Outfit instead triggers a small taunt wherein he adjusts his hat and scoffs. If the player reloads 4 times in a row, a more dynamic animation will play of Leon tossing and catching his hat.

The gun Killer7 is a reference to a game by the same name, produced by Shinji Mikami and published by Capcom. A character in Killer7 used a similar looking gun.

Before the events of the game, the villagers had their own children. According to the Biohazard 4: Incubate DVD, there were no children in the game because they were killed by the Plaga Virus.

Most of the usable guns in the game are real life guns under a nickname.

Default "Silver Ghost handgun" = H&K USP/S&W Sigma/Ruger P85 hybrid
Punisher = FN Five-seveN
Red9 = Mauser C96 (Red9 was an actual nickname for Mauser variants that handled 9mm bullets)
Blacktail = Springfield Armory XD
Matilda = Heckler & Koch VP70M (The very same pistol that Leon used in RE2, with its stock attachment and burst fire mode active)

Shotgun = Remington 870
Riot Gun = customised Benelli M3 Super 90
Striker = Armsel Protecta ("Striker" was the gun's original production name)
Ada's Shotgun = Mossberg 500

Rifle = Springfield M19031A1
Semi-auto Rifle = Heckler & Koch SL8

Broken Butterfly = S&W Schofield Model 3/Colt Single Action Army hybrid
Killer 7 = Colt M1911 magnum variant with an AMT Hardballer-style laser sight
Handcannon = Smith & Wesson Model 500

TMP = Steyr TMP. HUNK uses a customised variant resembling the Brugger & Thornet MP-9
Chicago Typewriter = Thompson submachine gun. Leon normally uses the M1A1 Army service variant while Ada and Mobster outfit Leon use the famous 1921 drum magazine version. "Chicago Typewriter" was one of many famous real life nicknames for the Thompson.

Rocket Launcher = RPG-7

The only truly fictional weapons are the Plaga Removal Laser 412, the Mine Thrower and the Bowgun.

There are a few possible references to the 1994 film "The Professional", starring Jean Reno. Jean's character's name in the film was "Leon", an unlockable pistol in RE4 is called "Matilda", after the 12 year old female protagonist of the film, and Resident Evil 4's hardest difficulty setting is named "Professional."

The unlockable Matilda pistol is actually the customised Heckler & Koch VP70 handgun Leon used in Resident Evil 2. Leon could find a stock and attachment for his VP70 pistol in RE2 that allowed him to fire bursts.

There is an early build of Resident Evil 4 that was completely scrapped, now referred to as Resident Evil 3.5. In the trailers and gameplay footage released for the game, Leon battled possessed dolls, a fog monster that resembles Uroboros, and a recurring enemy called Hookman.

Some elements used in Resident Evil 3.5 include Quick Time Events, some sort of secondary weapon for fighting the dolls, and a blue screen filter for battle sequences.

Leon was originally supposed to be rescuing Sherry Birkin in RE4 and the story was supposed to tie in with what Wesker said in Wesker's Report: "Sherry is safe in our hands". That part was, however, removed from both the game and the 10th anniversary edition of Wesker's Report.

After the Credits of Resident Evil 4, in the "copyrights" section, it says "This game is protected under the laws of Raccoon Police Department. Unauthorized duplication, distribution or exhibition may result in civil liability and criminal prosecution by an appropriate S.T.A.R.S. member. And then some."

Robert Kendo's brother, Joe Kendo, is hinted to still be alive, as the words "Kendo Custom Shop" have been engraved in Leon's gun, the Silver Ghost.

Should the player repeatedly shoot the lake just before battling Del Lago, the monster will burst out of the lake and eat Leon in one gulp, killing him instantly. The HD and Steam editions of the game award the player with the trophy/achievement "Don't Shoot The Water!" for doing this.

After the abandoned house scene where Leon is knocked out by Chief Mendez, he awakens without his jacket without explanation. In "Separate Ways"' second chapter, Ada comes across a Ganado wearing Leon's jacket.

Devil May Cry started out as Resident Evil 4. Producer Shinji Mikami felt it strayed too far from the series' survival horror roots and gradually convinced all the staff members to make it an independent game. The story was eventually rewrote to be set in a world full of demons and the hero's name was changed to Dante.