Because all of the Beatles' master recordings were originally recorded on 4-track and 2-track equipment, multiple instruments shared the same tapes. In order for the music to work with Rock Band, Giles Martin, the son of George Martin who oversaw the original production of most of the Beatles' music, had to spend months using digital filters to create separate tracks for each instrument.

According to the game's music producer Giles Martin, Paul McCartney gave himself the role of "executive tweaker" when working on the game. Martin remarked "the better title might be watchdog."

Every individual digital track made from the Beatles master recordings that were sent to Harmonix to make The Beatles: Rock Band were low-fidelity copies imprinted with static. This was a security measure to assure that The Beatles' music wouldn't be sampled by other artists in the event that the recordings were leaked.

These low-quality copies were used throughout development, and replaced with high-quality versions for production.

Before starting a song in any of the Studio Stages, the voices of the members of The Beatles practicing and talking to each other can be heard. These are actual recordings of the band members that were never released to the public before.

Plans for a Beatles-themed Rock Band game happened before the first Rock Band game was ever released, thanks to a meeting between Dhani Harrison (George Harrison's son) and MTV executive Van Toffler at a Christmas party in 2006.