An early version of the game's logo can still be found on the game's disc.

In the Advanced Extra 10 level "Teapot", a 3D model known as the "Utah Teapot" is used.

Surgeons at Florida Hospital Celebration Health played the game as a warm-up in order to improve performance on laparoscopic surgery.

Dr. Bad Boon's speech is actually reversed.

In Super Monkey Ball 2, beginner stage 3 "Bumpy" contains small, seemingly random bumps throughout the stage. From above, the bumps are revealed to be braille, and they read "Hi! This is Jamad. That's right. Braille alphabet. I respect you!!" "Jamad" is a nickname for Junichi Yamada, the game's stage design director.