At one point in development, it was planned for the Working Joe enemy to quote lines from William Shakespeare in a malevolent manner. However, it was ultimately decided it didn't fit with the tone of the game and the few recorded lines were cut from the game.
William Hope, who played Lt. Gorman in the Aliens film, voices Marshal Waits in the campaign. The character is also modeled after his physical appearance.
There are origami unicorns placed throughout the levels. These unicorns are a reference to the film "Blade Runner", directed by the original Alien movie director, Ridley Scott.