The game features a variety of crewman that are original characters not featured on the TV show Star Trek: The Next Generation. These characters were named after and modeled in the likeness of the developers of the game. One character, Ed Semrad, is actually based on the Electronic Gaming Monthly magazine editor of the same name.

Several still images from the game are digitized screen captures from various episodes of the television series. For example, the still frame of Captain Picard in his ready room is from the episode "Lonely Among Us". The image of Dr. Crusher healing an injured crewmember is from "The Battle", while the still frame of the crew in the observation lounge is based upon a scene from the episode "The Neutral Zone", with changes to both Troi and Worf's uniforms, as well as the addition of Riker's beard.