In the 2023 film "The Super Mario Bros. Movie", Mario and Luigi's family are prominently featured. This includes:

• Their Father, voiced by Charles Martinet (and bears a resemblance to Talon from The Legend of Zelda: Ocarina of Time)
• Their Mother, who previously appeared in "The Super Mario Bros. Super Show!" and is voiced here by Jessica DiCicco
• Uncle Arthur, voiced by John DiMaggio
• Uncle Tony, voiced by Rino Romano (and was coincidentally mentioned in live-action segment "Glasnuts" from the TV series "The Super Mario Bros. Super Show!")
• Aunt Marie, who's presumably married to Uncle Arthur
• An unnamed niece, who's most likely Marie and Arthur's daughter, being a first cousin once removed that's referred to as a "(second) niece"
• An unnamed grandfather, who's implied by the co-director Aaron Horvath to previously have been a boxer and wears an outfit similar to Luigi in the 1983 commercial for the Atari ports of Mario Bros.

Interestingly, all of the credited voice actors for the family are all of Italian descent to stay true to the family's Italian heritage.

According to Shigeru Miyamoto, the design of the family were based on character design sheets that former Nintendo illustrator Yoichi Kotabe drew decades before the film began production.