During the game's first case "Turnabout Memories", defense attorney Marvin Grossberg makes several references to his hemorrhoids acting up, with one being that they are "doing the Harlem Shake". While the English localization of the game wrote in several pop culture references and memes that were popular at the time of its release in 2007, this reference to the Harlem Shake was not a reference to the 2013 viral dance since it did not exist yet, but was actually a reference to the pre-existing Harlem Shake dance that first originated in New York City in 1981. However, according to the official Ace Attorney series timeline, Trials and Tribulations takes place in 2019, and in the events of that game, the Turnabout Memories case takes place five years beforehand in 2014, which is coincidentally one year after the Harlem Shake meme went viral. This makes it plausible for unaware players to assume that Grossberg is referencing the viral dance in the time when it was relevant, unintentionally predicting the meme's popularity and ostensibly inflating the number of meme references in the English script.

Alternatively, the German translation of the game instead has Grossberg say his hemorrhoids are doing "den Ententanz", also known as the Chicken Dance, another fad dance originating from Switzerland in the 1950's.