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In chapter 5, there is a turret that you can rescue for being "different." This turret (named the Oracle Turret by fans and programers) will spout off some several cryptic messages that relate to the later in the game:
• "Get mad. Don't make lemonade." - This is a quote from Cave Johnson.
Spoiler:"Prometheus was punished by the gods for giving the gift of knowledge to man. He was cast into the bowels of the earth and pecked by birds." - This is referencing how Wheatly is given all of GLaDOS's power and turns GLaDOS into a potato, throws her and you into the underground labs, and GLaDOS is pecked at by a bird.
Spoiler:"It won't be enough." - This reveals that the paradox will not work on Wheatly.
Spoiler:"The answer is beneath us." - This refers to the underground labs.
Spoiler:"Her name is Caroline." - Caroline being GLaDOS's real name.
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In one of Ratman's rooms is a radio blaring strange noises. This "noise" is actually a SSTV signal. If you decode it, it will show an image from the end of the game.
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During the "Bring Your Daughter to Work Day" science-fair, you will find a giant potato that is growing to the ceiling. If you look around the potato, you will find that it was made by Chell and she used a "special ingredient from daddy's work." This indicates that Chell was the daughter of an Aperture Science employee.
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Attachment During the development of the game, the team stated that there would be no cake jokes, as they were tired of the "The Cake Is A Lie" meme. Eventually, it turns out that they lied about this, as they "couldn't resist putting in just one."
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Attachment Because of the failure of the attempted meme of Hoopy the Hoop in Portal, VALVe included the model of Hoopy the Hoop in 3 spots in Portal 2: Near the deactivated GLaDOS in Chapter 1, In Chapter 2 Chamber 2, and behind a desk in a Ratman Den.
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In the Spanish version of Portal 2, at the end of chapter 5, when Wheatley takes over GLaDOS' body, Wheatley tries to show his intelligence to Chell by using the translator and in doing so, winds up saying, "It seems that you are using this translation too incorrectly. Please check the manual."
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Attachment During development, Cave Johnson's "secretary" was a scientist named Greg. But, Valve realized that they would have to hire another voice actor. In order to cut costs and enticed to create an origin for GLaDOS, Greg was cut and Caroline was added. Greg was later used as Cave Johnson's new partner in the Perpetual Testing Initiative DLC.
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Attachment There are unused audio files in Portal 2 that suggest there was once a scene where Caroline was forcibly transformed into GLaDOS.

'No listen to me! Sir, I do not want this.'
'I do not want this!'
'Mr. Johnson, I don't want this!'
'Cave, I'm begging you, don't! Please!'
'NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!'
*GLaDOS voice* 'I am awake. And I am going to kill you all.'
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Early in development, Portal 2 was planned to feature no portals at all. Valve focused on a new gameplay mechanic called "F-Stop", and though the new mechanic received some positive feedback, every single play tester was disappointed with the absence of portals. Valve then revised the game to include portals.
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