Collection: Mario Kart
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In "The Powerpuff Girls" episode titled "The Powerpuff Girls Rule!!!", the Mario Kart series is referenced during a scene that has the titular girls and Professor Utonium race against the villains in go karts, where he presses a button that fires a green turtle shell at Mojo-Jojo in a similar fashion to the shell items.
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According to Tesla CEO Elon Musk, a Mario Kart game that could be played while inside their cars was once proposed to Nintendo, only to be turned down as they refused to license the series to Tesla.
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In the episode "Bizarre Bazaar" of the animated series "Amphibia", during a race that Anne and Sprig have against their opponent, he uses a blue-shelled flying beetle with spikes that automatically chases and hits Anne and Sprig as they take the lead, after which Sprig comments "Well that didn't feel balanced." This is a blatant reference to the powerful Blue Shell item from the Mario Kart series.