Nightwing's Regime costume is Damian Wayne. This makes Nightwing one of several characters to have different people as alternative costumes. Spoiler:It is revealed during the game that the alternate dimension version of Damian killed Dick Grayson, the current true Nightwing, took his mantle and joined Superman's regime.

The other characters with different people as costumes are Green Lantern (with a John Stewart costume) and Superman's Cyborg outfit (which is actually a character named Hank Henshaw.)

Following the game's release in 2013, it was featured on the "Clueless Gamer" segment on comedian Conan O'Brien's Conan talk show. During this segment, O'Brien was able to play as Superman with the "Flaming C" costume. "Flaming C" was a character co-created by O'Brien and DC Comics artist Bruce Timm, modeled loosely on O'Brien himself. When asked about the Flaming C skin in 2021, game director Ed Boon confirmed that a build of the game with the costume "exists somewhere", but the costume was never made publicly available.