Very unusual for a LEGO adventure game, this game completely lacks any collectible Gold Bricks.
Due to the Instruction manual mentioning a "young Tim", and the DS version having a Tim Drake character, the primary Robin in this game is most likely Tim Drake.
The Military Policeman playable character, who never appears in any level of the game, actually reuses assets from the Desert Enemy Soldier characters from LEGO Indiana Jones: The Original Adventures.
A LEGO spoof of the rather graphic Rembrandt painting "The Anatomy Lesson of Doctor Nicolaes Tulp" can be found in The Joker's Masterpiece level.
In Free Play mode of the Villains level "An Enterprising Theft", the player can build and then ride the robotic dog companion K-9 from the science-fiction series Doctor Who.