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In the game's credits, one of the comments posted by one of the game's developers, Ashley Rochelle, reads "Mycaruba". This is a reference to the let's play web series Game Grumps and the meme from their Out of This World playthrough.
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The side mission Sub-Level 13 in Borderlands: The Pre-Sequel has many references to the characters, creators, and elements from the film Ghostbusters.
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The DJs in Moxxi's Bar, DJ Boom and DJ Rang, are clearly based on music duo Daft Punk.
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The mission "These are the Bots" pays homage to the classic film Star Wars Episode IV: A New Hope in multiple ways. In the mission, the player must help two robots, ICU-P and RT-FC to deliver a message to a man named Toby Van Adobe. In the beginning of Star Wars Episode IV, C-3PO and R2-D2, two droids, must deliver a message to jedi Obi-Wan Kenobi from Princess Leia. ICU-P's color scheme is similar to C-3PO's and RT-FC is similar to R2-D2's. The mission name is also similar to the famous line "These aren't the droids you're looking for" said in the film.
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On the desk found in Jack's office is a photograph Spoiler:of a young Angel from Borderlands and Borderlands 2.
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In the subconcious of the Claptastic Voyage DLC, you can find a partially buried Big Daddy from the Bioshock series in a pool of water.
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In the Claptastic Voyage DLC, there is a talking Legendary Shield called "M0RQ". The shield is a reference to the show "Mork & Mindy", and the voice from the shield sounds remarkably similar to Robin Williams, who starred in the show.
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In Spoiler:Tycho's Ribs, the entrance to the Vault, if the player goes up a specific eridian elevator into a pipe, noted by not being blocked by an energy shield, they will be transported to a Super Mario Bros. themed area.
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