There's a building with the developer's name, "Beenox".

In Chapter 4 when Spider-Man looks for Vermin in the sewers, Spider-Man asks Vermin if he is going to hang out with his "adopted turtle sons and eat pizza", a reference to the Teenage Mutant Ninja Turtles.

Stan Lee, creator of Spider-Man, makes a voice cameo in the game. He voices the character Stan, who is said to be popular for his "amazing story telling", "original works of fictions" and "numerous appearances in Hollywood productions". Stan's appearance is also based on Stan Lee.

There are a pair of Guitar Hero controllers in the windows of the pawn shops in the game.