The Two Cities update introduced two new maps for Mann vs. Machine: Rottenburg and Mannhattan.

Mannhattan introduced a new kind of robot called "GateBots" that attempt to gain control of spawning gates on the map instead of carrying the bomb. These robots can be identified by the yellow GateBot lights they wear. Due to a bug, it was temporarily possible for players to craft GateBot lights.

Valve replaced the crafted GateBot Lights with an item called "Glitched Circuit Board". According to the item's description, the lights self-destructed after falling into human hands.