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In the Japanese version, a small description/name will be displayed once you select a Robot Master.
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In the Japanese version of the game after Mega Man beats Dr. Wily and Spoiler:Wily says that robots cannot harm humans, Mega Man answers with "....." (which suggests that Mega Man is programmed with Asimov's Rules of Robotics). In the American version of the game, Mega Man says "I am more than a robot!! Die Wily!!" implying that Mega Man fully intended to kill Dr. Wily and break the Laws.
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In Shade Man's stage, right before you fight him, you will encounter Bass. In the SNES version he says "Damn!", while in the Anniversary Collection, it was changed to "Darn!". This was most likely done to avoid a Teen rating for the game (as the E10+ rating had not been made at the time of the original release).
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The theme from Ghosts 'n Goblins will play during Shade Man's stage if B is held while selecting the level.
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During the intro sequence, Dr. Wily is mostly covered by other graphics. Extracting his sprite from the game will show that he has distinct breasts. The orientation of his shoulders and arms means they couldn't be pectoral muscles. This could simple be poor rendering by the artist but given the precise nature of pixel art, it is more likely be intentional or possibly a joke by the developers.
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