In the basement of the Helicarrier, a character will ask the player to help them clear out some snakes, saying,



S.H.I.E.L.D. Director Nick Fury's appearance in the Marvel Ultimate line of comic books was based on Samuel L. Jackson, and he has played the role in a number of Marvel movies. Samuel L. Jackson also played the part of Neville Flynn, an FBI agent in the film "Snakes on a Plane", in which he exclaims, "That is it! I have had it with these mother-fucking snakes, on this mother-fucking plane!"
The opening line is also likely a reference to Indiana Jones, with the same line appearing in the 1981 movie, Raiders of the Lost Ark.