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During the second cutscene, Qwark's recording mentions pressing 1 if being attacked by radioactive amoeboids. This is a reference to Blackwater City in Ratchet & Clank, and the Qwark Vid-Comic in Ratchet & Clank: Up Your Arsenal, when amoeboids attacked Blackwater City.
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On top of the IRIS Super Computer Tube is a teddy bear sitting near one of the rods.
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Every enemy in the game has their own dance moves when they're hit with the "Groovitron" weapon.
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During the fight with The Plumber on Planet Sargasso he will say, "Almost didn't recognize you there in high-def.", which is a reference to the improvement in graphics from the games on the PlayStation 2 to those on the PlayStation 3.
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