Very early on in the game, if the player makes certain unorthodox decisions in the Kara storylineSpoiler: it is possible to have both Kara and Alice die extremely early. If the player goes to the Main Menu right after doing so, Chloe will slightly break the fourth wall and scold the player for "letting them die". She will then tell the player that the "lives of these androids are in your hands".
Contributed by PirateGoofy